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The MiniSeq Swift designed a recipe modify that makes use of the grafted oligo with the i5 index priming, so it can be study during the ahead orientation comparable to a MiSeq.
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When demultiplexing, the ultimate sample sheet provided to the FASTQ technology application must contain the i5 index in the correct orientation for that System. On the other hand, most Illumina sample sheet generation software handles this quickly.
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Index two: the i5 index column is during the reverse complement orientation (would be the reverse enhance of your i5 indexes during the sample sheet).
As a world corporation that spots large value on collaborative interactions, immediate supply of methods, and delivering the highest amount of excellent, we try to fulfill this problem. Illumina modern sequencing and array systems are fueling groundbreaking enhancements in everyday life science study, translational and purchaser genomics, and molecular diagnostics.
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Library prep, enrichment, and probe panel reagents have to be purchased separately. Appropriate library prep kits: